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Project:OD600 Measurement: Difference between revisions
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In the molecular biology of bacteria, OD600 is often used to estimate the current growth phase of a bacteria or other cells in suspension. It means the "optical density of the sample to light of 600nm". The measured OD600 gives an estimate of the concentration of the cells in the liquid, which can be used to estimate the growth phase of the population. 600nm is in the visible wavelength an is approximately orange. | In the molecular biology of bacteria, OD600 is often used to estimate the current growth phase of a bacteria or other cells in suspension. It means the "optical density of the sample to light of 600nm". The measured OD600 of the sample relative to a plain reference sample, gives an estimate of the concentration of the cells in the liquid, which can be used to estimate the growth phase of the population. 600nm is in the visible wavelength an is approximately orange. | ||
The basic idea is to take a sample of plain media, and a sample of media in which bacteria has been growing. Initially the levels of bacteria will be very low, and the amount of light absorbed by the 2 samples will be similar. | The basic idea is to take a sample of plain media, and a sample of media in which bacteria has been growing. Initially the levels of bacteria will be very low, and the amount of light absorbed by the 2 samples will be similar. | ||
[[File:File:OD600-pictore.png|500px]] | |||
[[File:14853028140 6a23c3757f k.jpg|300px]] | [[File:14853028140 6a23c3757f k.jpg|300px]] | ||
