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Project:OD600 Measurement: Difference between revisions
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[[File:Growthcurve.jpeg|300px]] | [[File:Growthcurve.jpeg|300px]] | ||
== Wavelengths of light == | |||
600nm is in the visible wavelength an is approximately orange. These are some 605nm LEDs from ebay. 600nm light is preferred to UV for example, because its much less phototoxic at those energies. It is also a wavelength at which organic materials readily absorb light. | 600nm is in the visible wavelength an is approximately orange. These are some 605nm LEDs from ebay. 600nm light is preferred to UV for example, because its much less phototoxic at those energies. It is also a wavelength at which organic materials readily absorb light. | ||
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[[File:27030-004-293E0372.jpg]] <br /> | [[File:27030-004-293E0372.jpg]] <br /> | ||
(Image: http://kids.britannica.com/comptons/art-57434/When-white-light-is-spread-apart-by-a-prism-or) | (Image: http://kids.britannica.com/comptons/art-57434/When-white-light-is-spread-apart-by-a-prism-or) | ||
== Commercial Spectrophotometers == | |||
The procedure is normally carried out using a visible light capable spectrophotometer like this one; | The procedure is normally carried out using a visible light capable spectrophotometer like this one; | ||
