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E. coli GFP transformation: Difference between revisions
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→E coli, PUC19, ampicillin selection
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We're interested in doing genetic modification on micro-organisms at the London Hackspace. Ideas for stuff to do: | We're interested in doing genetic modification on micro-organisms at the London Hackspace. Ideas for stuff to do: | ||
== E coli | == E coli transformed with PUC19 using ampicillin selection == | ||
=== 28 mar 15 === | === 28 mar 15 === | ||
* 2ml ecoli culture (bottle on shelf) incubated in 200ml LB (bottle in fridge) | |||
* -ve control is untransformed ecoli from same culture put on LBA | |||
* +ve control is transformed ecoli from same culture put on LB plates | |||
* Transform ecoli with PUC19 using electroporation | |||
* Plate onto LBA. | |||
== GFP transformation == | == GFP transformation == | ||
