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E. coli transformation: Difference between revisions
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Mycoplasma (talk | contribs) (Created page with "= E coli transformed with PUC19 using ampicillin selection and electroporation = See [here](https://docs.google.com/document/d/1x5DkcQTWwBIKxbLGHKAjH2Sw1wdQL2B12tLCeF21b1c/ed...") |
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= E coli transformed with PUC19 using ampicillin selection and electroporation = | = E coli transformed with PUC19 using ampicillin selection and electroporation = | ||
See [ | See [https://docs.google.com/document/d/1x5DkcQTWwBIKxbLGHKAjH2Sw1wdQL2B12tLCeF21b1c/edit?usp=sharing here] for protocol, successfully used by Tom H. | ||
=== 28 mar 15 === | === 28 mar 15 === | ||
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* Plate onto LBA. | * Plate onto LBA. | ||
* No e coli survived on plates, whether with Amp or not. Could be various reasons. | * No e coli survived on plates, whether with Amp or not. Could be various reasons. | ||
=== 11 Apr 15 === | |||
* Following main gdocs protocol above. Initial 2x10ml cultures started off at 13:00. | |||
= GFP transformation = | = GFP transformation = | ||
