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E. coli transformation: Difference between revisions
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* Following main gdocs protocol above. Initial 2x100ml cultures started off at 14:40. | * Following main gdocs protocol above. Initial 2x100ml cultures started off at 14:40. | ||
* Ended up with some growth on control amp plate as well as transformed amp plate. Will do colony PCR and check if it worked with M13 | |||
=== 21 Apr === | |||
* Took 1 colony from PUC19 dh5a plate and mixed with 250ul RO water | |||
* Incubated at 98C for 15 minutes, then hold at 10C (boiling program in PCR machine) - to lyse the cells | |||
* Put on 2 PCRs - one with M13 primers (for PUC19), one with S16 primers (for any bacteria), then hold for 4C for 20 hours. | |||
* From top - ladder, M13, S16 | |||
=== 22 Apr === | |||
= GFP transformation = | = GFP transformation = | ||
