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SPLiCE: Difference between revisions
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For our first attempt we used the Quick 50ul reaction from above and a higher concentration of insert (10:1), electroporated using the second protocol (see electroporation protocol) using electrocompetent cells kindly donated by the Birkbeck iGEM team. The reaction was a success and we had pink colonies on every plate! | For our first attempt we used the Quick 50ul reaction from above and a higher concentration of insert (10:1), electroporated using the second protocol (see electroporation protocol) using electrocompetent cells kindly donated by the Birkbeck iGEM team. The reaction was a success and we had pink colonies on every plate! | ||
[[File:Sheistheone|thumbnail]] | [[File:Sheistheone.jpeg|thumbnail]] | ||
. For a first dry run we call it a success :) <br> | . For a first dry run we call it a success :) <br> | ||
We obtained 1 colony in the plate containig 50ul of recovered cells after electroporation, 3 colonies on the 100ul and 11 colonies in the 350ul. We used this protocol to successfully make a new part BBa_K1845000 which we submitted to the registry. <br> | We obtained 1 colony in the plate containig 50ul of recovered cells after electroporation, 3 colonies on the 100ul and 11 colonies in the 350ul. We used this protocol to successfully make a new part BBa_K1845000 which we submitted to the registry. <br> | ||
