HS Medium: Difference between revisions
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== Protocol == | == Protocol == | ||
# Using a balance weigh out the following and transfer to a 1 litre screwcap bottle: | # Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: | ||
#* 20g D-glucose | #* '''20g D-glucose''' | ||
#* 5g peptone | #* '''5g peptone''' | ||
#* 5g yeast extract | #* '''5g yeast extract''' | ||
#* 1.15g citrate | #* '''1.15g citrate''' | ||
#* 2.7g disodium phosphate | #* '''2.7g disodium phosphate''' | ||
#* (15g agar if making solid medium for plates) | #* (15g agar if making solid medium for plates) | ||
# Half fill the bottle with water | # Half fill the bottle with water | ||
# Mix until all the additives are dissolved | # Mix until all the additives are dissolved | ||
# Adjust pH | # Adjust to '''pH 6.0''' using the pH meter and adding drops of NaOH | ||
# Make up to 1 litre with water | # Make up to 1 litre with water | ||
# Autoclave | # Autoclave | ||
Revision as of 01:38, 13 September 2014
Info
Also known as Hestrin–Schramm medium. Used for growing Gluconacetobacter hansenii.
Protocol
- Using a balance weigh out the following and transfer to a 1 litre screwcap bottle:
- 20g D-glucose
- 5g peptone
- 5g yeast extract
- 1.15g citrate
- 2.7g disodium phosphate
- (15g agar if making solid medium for plates)
- Half fill the bottle with water
- Mix until all the additives are dissolved
- Adjust to pH 6.0 using the pH meter and adding drops of NaOH
- Make up to 1 litre with water
- Autoclave
Notes
none.
