HS Medium: Difference between revisions
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== Info == | == Info == | ||
Also known as Hestrin–Schramm medium. Used for growing [[Gluconacetobacter hansenii]]. | Also known as Hestrin–Schramm medium. Used for growing ''[[Gluconacetobacter hansenii]]''. | ||
== Protocol == | == Protocol == | ||
Revision as of 01:41, 13 September 2014
Info
Also known as Hestrin–Schramm medium. Used for growing Gluconacetobacter hansenii.
Protocol
- Using a balance weigh out the following and transfer to a 1 litre screwcap bottle:
- 20g D-glucose
- 5g peptone
- 5g yeast extract
- 1.15g citrate
- 2.7g disodium phosphate
- (15g agar if making solid medium for plates)
- Half fill the bottle with water
- Mix until all the additives are dissolved
- Adjust to pH 6.0 using the pH meter and adding drops of NaOH
- Make up to 1 litre with water
- Autoclave
Notes
none.
