HS Medium: Difference between revisions
From London Hackspace Wiki
m (→Notes) |
|||
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
== Info == | == Info == | ||
Also known as Hestrin–Schramm medium. Used for growing [[Gluconacetobacter hansenii]]. | Also known as Hestrin–Schramm medium. Used for growing ''[[Gluconacetobacter hansenii]]''. | ||
== Protocol == | == Protocol == | ||
# Using a balance weigh out the following and transfer to a 1 litre screwcap bottle: | # Using a balance weigh out the following and transfer to a '''1 litre''' screwcap bottle: | ||
#* 20g D-glucose | #* '''20g D-glucose''' | ||
#* 5g peptone | #* '''5g peptone''' | ||
#* 5g yeast extract | #* '''5g yeast extract''' | ||
#* 1.15g citrate | #* '''1.15g citrate''' | ||
#* 2.7g disodium phosphate | #* '''2.7g disodium phosphate''' | ||
#* (15g agar if making solid medium for plates) | #* (15g agar if making solid medium for plates) | ||
# Half fill the bottle with water | # Half fill the bottle with water | ||
# Mix until all the additives are dissolved | # Mix until all the additives are dissolved | ||
# Adjust pH | # Adjust to '''pH 6.0''' using the pH meter and adding drops of NaOH | ||
# Make up to 1 litre with water | # Make up to 1 litre with water | ||
# Autoclave | # Autoclave | ||
== Notes == | == Notes == | ||
1.15g citrate can be replaced with 25ml of lemon juice. | |||
[[Category:Biohacking]] | |||
Latest revision as of 20:11, 14 October 2014
Info
Also known as Hestrin–Schramm medium. Used for growing Gluconacetobacter hansenii.
Protocol
- Using a balance weigh out the following and transfer to a 1 litre screwcap bottle:
- 20g D-glucose
- 5g peptone
- 5g yeast extract
- 1.15g citrate
- 2.7g disodium phosphate
- (15g agar if making solid medium for plates)
- Half fill the bottle with water
- Mix until all the additives are dissolved
- Adjust to pH 6.0 using the pH meter and adding drops of NaOH
- Make up to 1 litre with water
- Autoclave
Notes
1.15g citrate can be replaced with 25ml of lemon juice.
