LBL04002: Difference between revisions
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Revision as of 09:54, 30 October 2014
The autoclave is a Melag EuroKlav 29V-S
The operating manual is here; http://www.eickemeyer.nl/images/products/2281/500032_Melag_Euroklav_23VS.pdf
Use the autoclave can be used for 2 main operations
1) sterilization of media, glassware and autoclavable equipment before a protocol
2) Deactivation of GMO, and GMO exposed waste and equipment at the end of the protocol
sterilization of liquid media
Use the
- Sterilize the load using the Quick-Programme (15 psi/121 C/15 mins)
Deactivation of liquid and solid GMO waste
- transport the waste to the autoclave in a non-drip autoclavable tray
- ensure there are no folds or containers which can trap air
- Sterilize the load using the Universal-Programme (2.1 bar/134 C/15 mins)
- once the programme has run, the waste should be transferred to the chemical kill bin, for final deactivation
