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| ==Gene detection experiment==
| | [[Category:Pledges]] |
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| At a guesstimated total cost of £200.
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| This would give us the immediate means to do gene detection (we will design [http://en.wikipedia.org/wiki/Primer_(molecular_biology) primers] to extract particular genes.) E.g.:
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| * Detection of genetic modifications (identify [http://en.wikipedia.org/wiki/Genetically_modified_food GM food])
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| * Detection of species ("lamb curry test": are you getting what you bought?)
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| * Detection of variants of species (e.g. detect known traits based on published gene sequences)
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| * We can also send the results to a [http://en.wikipedia.org/wiki/Sequencing sequencing lab] to get raw data back.
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| '''Pledges:'''
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| * martind £20
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| * ...
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| === Details ===
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| The money would provide us with enough materials for dozens to hundreds of experiments (depending on the particular item.)
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| For [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR]:
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| * Primers (£20)
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| * PCR Reagents (£40)
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| * Pipette & tips (£30)
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| * 0.5ml tubes (£10)
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| For [http://en.wikipedia.org/wiki/Electrophoresis electrophoresis]:
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| * Power source (100V, low hundreds of mA; we have this)
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| * Agarose (£30)
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| * TBE Tablets (£10)
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| * Ladder (£20)
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| * Loading buffer (£20)
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| * Gel red/SYBR safe (£30)
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| ==PCR machine / Thermal cycler==
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| ALL MONEY RECEIVED - THANKS! PLEASE DIRECT YOUR MONEY TO OTHER ITEMS ON OUR PLEDGE LIST.
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| We [http://groups.google.com/group/london-hack-space/msg/253a37314f29da95? won] the PCR machine for £120.
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| * <s>Nigel £30</s> - '''PAID £15'''
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| * <s>Gilda £40</s> - '''PAID £15'''
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| * <s>Irwin £15</s> - '''PAID £15'''
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| * <s>Lauren £15</s> - '''PAID £15'''
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| * <s>Martind £100</s> - '''PAID £15''', pls claim the rest from me once more purchases were made
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| * <s>Jonty £15</s> - '''PAID £15'''
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| * Radek £30
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| * <s>Bugs £30</s> - '''PAID £15'''
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| * Keith $100 USD
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| '''Total pledged: £275''' + $100 from the colonies
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| '''Total needed: £120'''
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| '''PAID: £105'''
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| ==Incubator==
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| This one has been bid on and should be OK, currently at £10
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| http://cgi.ebay.co.uk/220725833946
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| ...might need a bit of hacking, but we're good at that
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