Difference between revisions of "Flow Cytometer"

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{{EquipmentInfobox
 
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| name = Flow Cytometer <!-- Name of the item. -->
 
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| image = IMG 20130927 144739.jpg <!-- Image of the item. Leave with placeholder image if none exists. -->
 
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| model = Coulter EPICS ALTRA  <!-- Model -->
 
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| category = Equipment <!-- Main category. Please leave alone to keep item in this category -->
 
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| subcat = Biology<!-- Sub-category if one exists. Please check main listing to see other categories contained within the main one -->
 
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| status = Faulty<!-- Set to one of; Good working order, Faulty, Out of order, Under construction, Out of consumables, Scrapped, or Unknown -->
 
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| consumables = Sheath Solution, Cleaning fluid, sample tubes<!-- Any items used up in normal operation, such as; ink, paper, saw-blades, cutting disks, oil, etc.. -->
 
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| accessories = Unknown <!-- Any items associated with the equipment but not consumable, such as; drill bits, safety gloves, goggles, etc.. -->
 
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| reqtraining = Yes<!-- Is training required to use this equipment? Yes or no. -->
 
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| trainlink = Unknown <!-- If training is required, provide a link to training signup or contact page. Otherwise leave blank. -->
 
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| acnode = <!-- Is the equipment ACnode locked? -->
 
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| owner = LHS/biohackers <!-- Provide a link to owners members page if other than LHS -->
 
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| origin = Donated by Imperial College London<!-- If via pledge, please link to the completed pledge page on the wiki -->
 
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| location = Basement, PCB area/Darkroom<!-- Floor, room/zone and location within that area -->
 
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| maintainers = tolland<!-- NO LINKS PLEASE; it currently breaks the template. If someone is nominated as managing the upkeep of this item, please list them here. -->
 
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| template_ver = 1.1 <!-- Please do not change. Used for tracking out-of-date templates -->
 
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Imperial College donated a Flow Cytometer. The unit is currently sitting on 1.8m X 0.8m = 1.44 square meters of floor space in the PCB area of the basement.  
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<s>Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.</s>
  
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'''Status:''' Currently waiting for basement plumbing to complete, before proceeding with fixing the fluid-assembly at the front right of the instrument. It needs a dust cover, or some protection to access the laser cabinet.
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<s>'''Status:''' It's sustained damage to the sampling area, and we are missing some bits. Repairing is likely to be very $$$, so we are planning to dismantle it and use the optics and lasers on other projects.</s>
  
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There are a number of large external units some of which are for providing power and cooling to the large laser. (I do not anticipate that the largest laser will be tested for some time, so I hope to move these into out-of-the-way storage, under the worktop in the biolab)
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'''Status:''' The machine was too expensive to repair, and was too large to store and therefore has been scrapped. Several of the parts have been incorporated into other projects, or have been ebayed.
  
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There is a good description of how a flow cytometer works here; http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html
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The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space) and was previously moved to storage while we worked on it.
  
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It was donated as working, but in need of repair. There is quite a lot of damage due to corrosion, and to the sampling area which we are working on.
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There are a number of large external units some of which are for providing power and cooling to the large laser.  
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There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html
  
 
The machine requires a dust free environment due to it's use of lasers and optics.
 
The machine requires a dust free environment due to it's use of lasers and optics.
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This is a AV presentation describing the basics of the technology;
 
This is a AV presentation describing the basics of the technology;
  
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http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html
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http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html
  
  
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http://www.youtube.com/watch?v=y1waCEPpWPI
 
http://www.youtube.com/watch?v=y1waCEPpWPI
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[[Category:Biohacking]]

Latest revision as of 15:08, 2 January 2015


Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.

Status: It's sustained damage to the sampling area, and we are missing some bits. Repairing is likely to be very $$$, so we are planning to dismantle it and use the optics and lasers on other projects.

Status: The machine was too expensive to repair, and was too large to store and therefore has been scrapped. Several of the parts have been incorporated into other projects, or have been ebayed.

The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space) and was previously moved to storage while we worked on it.


There are a number of large external units some of which are for providing power and cooling to the large laser.

There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html

The machine requires a dust free environment due to it's use of lasers and optics.

I am currently looking to obtain a copy of the service manual.


Educationz

Harvard University extension school course on microfluidics, course notes

http://isites.harvard.edu/icb/icb.do?keyword=k41912&pageid=icb.page213804

Caltech course notes on microfluidics

http://thesis.library.caltech.edu/4796/3/02_Chapter_2.pdf


Cornell book on microfluidics

http://www.kirbyresearch.com/index.cfm/wrap/textbook/microfluidicsnanofluidics.html


Flow cytometry tutorial

This is a AV presentation describing the basics of the technology;

http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html


Practical Flow cytometry

This is an online version of the bible of Flow cytometry (Shapiro 2003)

http://www.coulterflow.com/bciflow/practical/book/index.html


Dako - flow cytometry

http://www.dako.com/08065_15dec05_guide_to_flow_cytometry.pdf

Microfluidics Links

Introduction to fluidics

http://www.youtube.com/watch?v=y1waCEPpWPI

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