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| == Operating the Spectrophotometer ==
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| {| class="wikitable"
| | The Biolab SOPs are now stored on github; |
| | | https://github.com/london-biohackers/biohackspace-sops/blob/master/index.rst |
| |Author: Tom Hodder
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| |Approved by: S. Thompson
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| |SOP No. LBL04001
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| |Signed:
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| |Signed:
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| |Effective from:
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| |-
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| |Date:
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| |Date:
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| |Last edited:
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| |}
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| '''1. Purpose'''
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| ** The following document describes the procedure for the operation of the UNICAM Helios Beta Spectrophometer.
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| [[File:14794946891 a616cdbabe z.jpg|300px]]
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| '''2. Scope'''
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| ** This instrument is used to meaure how much light is absorbed by a liquid sample. In full working condition this instrument supports a range of visible and UV wavelength of light.
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| '''3. Responsibilities'''
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| ** It is the responsibility of lab users to use this equipment in the manner described in this SOP.
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| ** It is the responsibility of lab users to report any incidents or technical problems with the equipment
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| ** It is the responsibility of the lab management to provide training, technical support and to ensure compliance with the operating procedures.
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| '''4. Materials'''
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| ** Quartz or plastic cuvettes with a optical path of 12mm
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| ** The equipment has replaceable UV and visible light bulbs
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| '''5. Related documents'''
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| ** https://wiki.london.hackspace.org.uk/view/Spectrophotometer
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| ** Software manual http://www.vtpup.cz/common/manual/PrF_biofyz_ThermoSpectronic_UV500_manual_EN.pdf
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| ** User Guide https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Mdemploi/UNI050_EN%202203_%20HELIOS%20SERIES_%20USER.pdf
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| '''6. Definitions'''
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| ** Cuvette: A small, transparent quartz vessel. This is what a sample is put into for testing in the UV - Visible Spectrophotometer.
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| '''7. Procedures'''
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| ** The spectrophotometer should be started with good time before its required as the initialization procedure takes ~15 mins
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| ** The user should ensure that the sample cuvette is clean before it is inserted into the device
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| [[Category:Biohacking]]
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