|
|
| Line 1: |
Line 1: |
| == Operating the Spectrophotometer ==
| |
|
| |
|
| {| class="wikitable"
| | The Biolab SOPs are now stored on github; |
| | | https://github.com/london-biohackers/biohackspace-sops/blob/master/index.rst |
| |Author: Tom Hodder
| |
| | |
| |Approved by: S. Thompson
| |
| | |
| |SOP No. LBL04001
| |
| | |
| |-
| |
| | |
| |Signed:
| |
| | |
| |Signed:
| |
| | |
| |Effective from:
| |
| | |
| |-
| |
| | |
| |Date:
| |
| | |
| |Date:
| |
| | |
| |Last edited:
| |
| | |
| |}
| |
| | |
| '''1. Purpose'''
| |
| | |
| ** The following document describes the procedure for the operation of the UNICAM Helios Beta Spectrophometer.
| |
| | |
| [[File:14794946891 a616cdbabe z.jpg|300px]]
| |
| | |
| '''2. Scope'''
| |
| | |
| ** This instrument is used to meaure how much light is absorbed by a liquid sample. In full working condition this instrument supports a range of visible and UV wavelength of light.
| |
| | |
| '''3. Responsibilities'''
| |
| | |
| ** It is the responsibility of lab users to use this equipment in the manner described in this SOP.
| |
| ** It is the responsibility of lab users to report any incidents or technical problems with the equipment
| |
| ** It is the responsibility of the lab management to provide training, technical support and to ensure compliance with the operating procedures.
| |
| | |
| '''4. Materials'''
| |
| | |
| ** Quartz or plastic cuvettes with a optical path of 12mm
| |
| ** The equipment has replaceable UV and visible light bulbs
| |
| | |
| '''5. Related documents'''
| |
| | |
| ** https://wiki.london.hackspace.org.uk/view/Spectrophotometer
| |
| ** Software manual http://www.vtpup.cz/common/manual/PrF_biofyz_ThermoSpectronic_UV500_manual_EN.pdf
| |
| ** User Guide https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Mdemploi/UNI050_EN%202203_%20HELIOS%20SERIES_%20USER.pdf
| |
| | |
| '''6. Definitions'''
| |
| | |
| ** Cuvette: A small, transparent quartz vessel. This is what a sample is put into for testing in the UV - Visible Spectrophotometer.
| |
| | |
| '''7. Procedures'''
| |
| | |
| ** The spectrophotometer should be started with good time before its required as the initialization procedure takes ~15 mins
| |
| ** The user should ensure that the sample cuvette is clean before it is inserted into the device
| |
| | |
| ** For OD600 selected the wavelength for 600nm.
| |
| | |
| ** run a reference sample of media only sample
| |
| | |
| ** run the sample cuvette
| |
| | |
| | |
| ** ensure the machine is switched off after use
| |
| | |
| | |
| [[Category:Biohacking]]
| |
