LBL04006

Using the Autoclave for Waste Deactivation

Author: Tolland	Approved by: S. Thompson	SOP No. LBL04006
Signed:	Signed:	Effective from:
Date:	Date:	Last edited:

1. Purpose This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for two main procedures:

   i) sterilization of media, glassware and autoclavable equipment prior to another protocol
  ii) Deactivation of GMOs, and GMO exposed waste and equipment.

2. Scope

This should be observed every time the autoclave is used within the LBL BioLab for GMO waste deactivation.

3. Responsibilities

The operator of the autoclave is responsible for their own safety and that of others in the vicinity during its use. The particular risks presented in using this piece of equipment are primarily due to the high temperatures and steam released during evacuation.

Upon removal of items from the autoclave be aware that they will likely still be hot, and care should be taken to check that glass or ceramic items are intact.

4. Materials

The autoclave requires a supply of RO water. If the water supply level falls below a minimum level, the operator will be required to top up the system.

5. Related documents

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6. Definitions

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7. Procedures

7.1 Sterilization of liquid media

7.1.1 Use the * Sterilize the load using the Quick-Programme (15 psi/121 C/15 mins)

7.2 Deactivation of liquid and solid GMO waste ==

7.2.1 Transport the waste to the autoclave in a non-drip autoclavable tray 7.2.2 Ensure there are no folds or containers which can trap air 7.2.3 Sterilize the load using the Universal-Programme (2.1 bar/134 C/15 mins) 7.2.4 once the programme has run, the waste should be transferred to the chemical kill bin, for final deactivation

8. Resources

- Operating manual: http://www.eickemeyer.nl/images/products/2281/500032_Melag_Euroklav_23VS.pdf