MindControlBacteria: Difference between revisions
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==Overview== | ==Overview== | ||
The goal of this project is to mind-control the expression of a recombinant gene in <i> E. coli</i> using a commercial EEG headset. It takes inspiration from the mind-controlled paramecia (@Genspace) and a recent paper on the mind-controlled transgene expression in mammalian cells (Nature comms.) | The goal of this project is to mind-control the expression of a recombinant gene in <i> E. coli</i> using a commercial EEG headset. It takes inspiration from the mind-controlled paramecia (@Genspace) and a recent paper on the mind-controlled transgene expression in mammalian cells (Nature comms.) | ||
==Types of control== | |||
"Neurofeedback" and "direct control" //expand on the definitions in the Nature comms. paper// | |||
== IPTG inducible system == | |||
===Overview==+ | |||
This approach consists in the control of an open syringe pump using a commercial EEG headset, based on a simple arduino hack that you can find at this website http://frontiernerds.com/brain-hack | |||
The open syringe pump is directly controlled by the arduino, and will only add IPTG to the media when a threshold of concentration/meditation is achieved. The IPTG is added (during log phase growth of the <i> E. coli </i>) to the media: the bacteria will produce GFP and fluoresce depending on your mind state. | |||
===Work so far=== | |||
====Wetlab==== | |||
- Transformed BBa_K731520 (IPTG inducible GTP, composed of the lacI repressor, lac operator and GFP coding sequence) </br> | |||
- Set up O/N culture IPTG+/- | |||
====Hardware==== | |||
Hacked Mindflex headset with Arduino to sniff the data. | |||
== Ontogenetically controlled system == | |||
==Future directions== | |||
- Get a better Headset (with this one very low noise/signal ration) </br> | |||
- Complete the genetic circuit for optogenetical mind-control of trangene expression </br> | |||
Revision as of 12:05, 25 May 2015
Mind Controlled Transgene Expression in E. coli
Overview
The goal of this project is to mind-control the expression of a recombinant gene in E. coli using a commercial EEG headset. It takes inspiration from the mind-controlled paramecia (@Genspace) and a recent paper on the mind-controlled transgene expression in mammalian cells (Nature comms.)
Types of control
"Neurofeedback" and "direct control" //expand on the definitions in the Nature comms. paper//
IPTG inducible system
===Overview==+ This approach consists in the control of an open syringe pump using a commercial EEG headset, based on a simple arduino hack that you can find at this website http://frontiernerds.com/brain-hack The open syringe pump is directly controlled by the arduino, and will only add IPTG to the media when a threshold of concentration/meditation is achieved. The IPTG is added (during log phase growth of the E. coli ) to the media: the bacteria will produce GFP and fluoresce depending on your mind state.
Work so far
Wetlab
- Transformed BBa_K731520 (IPTG inducible GTP, composed of the lacI repressor, lac operator and GFP coding sequence)
- Set up O/N culture IPTG+/-
Hardware
Hacked Mindflex headset with Arduino to sniff the data.
Ontogenetically controlled system
Future directions
- Get a better Headset (with this one very low noise/signal ration)
- Complete the genetic circuit for optogenetical mind-control of trangene expression
