User:Tolland

Is a biohacker also working on microcontroller and biolab automation projects. Is interesting in learning new fabrication techniques and tools.

contact email: tom@limepepper.co.uk

Stuff I made list

Video Microscopy

This is a video I made of the interior of a single cell dinoflagellate using a Samsung S2 cameraphone and a OMAX microscope from the Dinos in the biolab Dinos in the biolab

Bacteria Dividing (12hour time lapse) Bacteria dividing under microscope with focus controlled by stepper

Welding

MIG welded candlestick (done at the "One little girl and a can of gasoline" studio over in Clare street)

This trolley was originally MIG welded from steel tube for transporting a laser, but it's now donated to the space in the PCB area.

Biolab Equipment

I made a new keypad for this PCR machine (it works honest!);

For a while we had some bio-luminescent algae in the biolab, and this was their illumination box as they are photosynthetic algae

Algae Illuminator Lamp

Bio luminescent algeo require a "training" cycle of 12 hours of darkness, followed by 12 hours of light. They will then produce the maximum light output when agitated when in their "night time" phase. This is a device to optimally illuminate a flask of dinoflaggelates using an arduino controlled led lamp array.

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Biobot data logger and IRC bot

Biobot is a raspberry pi based data logger and IRC bot.

you can talk to it in #london-biohackers on freenode

http://webchat.freenode.net/?channels=london-biohackers

Biohacker microscope

This is a project to create a cheap mount for mobile phone cameras, and to transmit the live image using an android app to a desktop computer;

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Inverted microscope

A proof of concept of a cheap researcher microscope in an inverted configuration using a makerbeam frame system;

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Nasal flow data logger

A simple device to measure the relative left/right nasal flow using an arduino and differential air pressure component.

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Shaker Platform

many techniques for culturing microbes benefit from continuous agitation. This is a project to build a shaker platform out of readily available parts and drive the mechanism using the plate turner in a microwave over.

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OD600 handheld unit

This is a project to create a cheap hand held unit to determine the OD600 level of a biological sample. Typically this is used for determining mid-log phase of bacterial growth in molecular biology.

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I'd also like to resin cast some calibration blocks, so I can compare between equipment in a standard way;

http://www.myfluxcapacitor.com/plastic-resin-casting-guide

Laser Cutting

3d printed pi case from design on instructables

Lab Automation

I made a automatic pipettor with a stepper motor, the linear bearing was done on the 3-in-one lathe {{#ev:youtube|pEgEQ2Acyv8}}

Automated Pipettor for robot arm

The hackspace has a staubli robot arm. This is a project to create a automated pipettor driven by a stepper motor

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We have some components that can be used to improve this