LBL04002

Using the Autoclave for Media Preparation

Author: Tolland	Approved by: S. Thompson	SOP No. LBL04002
Signed:	Signed:	Effective from:
Date:	Date:	Last edited:

1. Purpose This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for two main procedures:

   i) sterilization of media, glassware and autoclavable equipment prior to another protocol
  ii) Deactivation of GMOs, and GMO exposed waste and equipment.

2. Scope

This should be observed every time the autoclave is used within the LBL BioLab.

3. Responsibilities

The operator of the autoclave is responsible for their own safety and that of others in the vicinity during its use. The particular risks presented in using this piece of equipment are primarily due to the high temperatures generated, steam and high pressures.

4. Materials

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5. Related documents

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6. Definitions

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7. Procedures

7.1 Sterilization of liquid media

7.1.1 Use the * Sterilize the load using the Quick-Programme (15 psi/121 C/15 mins)

7.2 Deactivation of liquid and solid GMO waste ==

7.2.1 Transport the waste to the autoclave in a non-drip autoclavable tray 7.2.2 Ensure there are no folds or containers which can trap air 7.2.3 Sterilize the load using the Universal-Programme (2.1 bar/134 C/15 mins) 7.2.4 once the programme has run, the waste should be transferred to the chemical kill bin, for final deactivation

8. Resources

- Operating manual: http://www.eickemeyer.nl/images/products/2281/500032_Melag_Euroklav_23VS.pdf