LBL04001: Difference between revisions
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'''6. Definitions''' | '''6. Definitions''' | ||
** | ** Cuvette: A small, transparent quartz vessel. This is what a sample is put into for testing in the UV - Visible Spectrophotometer. | ||
'''7. Procedures''' | '''7. Procedures''' | ||
** | ** The spectrophotometer should be started with good time before its required as the initialization procedure takes ~15 mins | ||
** The user should ensure that the sample cuvette is clean before it is inserted into the device | |||
** | |||
[[Category:Biohacking]] | [[Category:Biohacking]] |
Revision as of 18:41, 12 January 2015
Operating the Spectrophotometer
Author: Tom Hodder | Approved by: S. Thompson | SOP No. LBL04001 |
Signed: | Signed: | Effective from: |
Date: | Date: | Last edited: |
1. Purpose
- The following document describes the procedure for the operation of the UNICAM Helios Beta Spectrophometer.
2. Scope
- This instrument is used to meaure how much light is absorbed by a liquid sample. In full working condition this instrument supports a range of visible and UV wavelength of light.
3. Responsibilities
- It is the responsibility of lab users to use this equipment in the manner described in this SOP.
- It is the responsibility of lab users to report any incidents or technical problems with the equipment
- It is the responsibility of the lab management to provide training, technical support and to ensure compliance with the operating procedures.
4. Materials
- Quartz or plastic cuvettes with a optical path of 12mm
- The equipment has replaceable UV and visible light bulbs
5. Related documents
6. Definitions
- Cuvette: A small, transparent quartz vessel. This is what a sample is put into for testing in the UV - Visible Spectrophotometer.
7. Procedures
- The spectrophotometer should be started with good time before its required as the initialization procedure takes ~15 mins
- The user should ensure that the sample cuvette is clean before it is inserted into the device