LBL04001

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Operating the Spectrophotometer

Author: Tom Hodder Approved by: S. Thompson SOP No. LBL04001
Signed: Signed: Effective from:
Date: Date: Last edited:

1. Purpose

    • The following document describes the procedure for the operation of the UNICAM Helios Beta Spectrophometer.

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2. Scope

    • This instrument is used to meaure how much light is absorbed by a liquid sample. In full working condition this instrument supports a range of visible and UV wavelength of light.

3. Responsibilities

    • It is the responsibility of lab users to use this equipment in the manner described in this SOP.
    • It is the responsibility of lab users to report any incidents or technical problems with the equipment
    • It is the responsibility of the lab management to provide training, technical support and to ensure compliance with the operating procedures.

4. Materials

    • Are there any extra materials required, in the case of operating an instrument, peice of equipment or in a molecular biology procedure this might be consumables, reagents, chemicals or other instrumentation, e.g pipets. Any special PPE can be mentioned here.

5. Related documents

    • Are other SOPs (or other documents within our framework) related to this procedure. If so then listing them here can be useful, for example the media prep SOP might be relevant when carrying out the transformation procedures and it saves time to link it here rather than describe the preparation of media in every procedure where it is used (bit like an include/import/use lol).

6. Definitions

    • Any useful glossary stuff

e.g: SOP = Standard Operating Procedure

7. Procedures

    • 7.1 The actual steps of the procedure go here ...
       7.1.1 They can be embeded/indented as much as is reasonably helpful to describe the procedure step by step
    • 7.2 It should be written in such a way that it would help anyone new to the lab to perform the procedure without having prior expertise.

8. Resources

    • This is where eternal useful documention can be linked, much like the references section in any academic paper.


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