Difference between revisions of "HS Medium"
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== Notes == | == Notes == | ||
| − | + | 1.15g citrate can be replaced with 25ml of lemon juice. | |
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[[Category:Biohacking]] | [[Category:Biohacking]] | ||
Latest revision as of 20:11, 14 October 2014
Info
Also known as Hestrin–Schramm medium. Used for growing Gluconacetobacter hansenii.
Protocol
- Using a balance weigh out the following and transfer to a 1 litre screwcap bottle:
- 20g D-glucose
- 5g peptone
- 5g yeast extract
- 1.15g citrate
- 2.7g disodium phosphate
- (15g agar if making solid medium for plates)
- Half fill the bottle with water
- Mix until all the additives are dissolved
- Adjust to pH 6.0 using the pH meter and adding drops of NaOH
- Make up to 1 litre with water
- Autoclave
Notes
1.15g citrate can be replaced with 25ml of lemon juice.