Difference between revisions of "LBA"
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Mycoplasma (talk | contribs) |
Mycoplasma (talk | contribs) |
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| − | * You want 100ug/ml, meaning 10mg in 100ml | + | * You want 100ug/ml ultimately [http://openwetware.org/wiki/Ampicillin for high copy plasmids], meaning 10mg in 100ml |
* So you can use ampicillin stock solution (1000x conc) at 1ul / ml. | * So you can use ampicillin stock solution (1000x conc) at 1ul / ml. | ||
==Make new stock== | ==Make new stock== | ||
| + | * Needs to be 1000x, so 100mg per ml | ||
Solubility rating is 50mg/ml, so make 1ml of stock. | Solubility rating is 50mg/ml, so make 1ml of stock. | ||
| Line 9: | Line 10: | ||
Microwave? | Microwave? | ||
| + | Add ampicillin at 48C | ||
Latest revision as of 22:41, 15 April 2015
- You want 100ug/ml ultimately for high copy plasmids, meaning 10mg in 100ml
- So you can use ampicillin stock solution (1000x conc) at 1ul / ml.
Make new stock
- Needs to be 1000x, so 100mg per ml
Solubility rating is 50mg/ml, so make 1ml of stock.
Plates
Microwave? Add ampicillin at 48C