Difference between revisions of "LBL04001"

Latest revision as of 20:15, 13 October 2015

The Biolab SOPs are now stored on github; https://github.com/london-biohackers/biohackspace-sops/blob/master/index.rst

@@ Line 1: / Line 1: @@
-== Operating the Spectrophotometer ==
-{| class="wikitable"
+The Biolab SOPs are now stored on github;
+https://github.com/london-biohackers/biohackspace-sops/blob/master/index.rst
-|Author: Tom Hodder
-|Approved by: S. Thompson
-|SOP No. LBL04001
-|-
-|Signed:
-|Signed:
-|Effective from:
-|-
-|Date:
-|Date:
-|Last edited:
-|}
-'''1. Purpose'''
-**  The following document describes the procedure for the operation of the UNICAM Helios Beta Spectrophometer.
-[[File:14794946891 a616cdbabe z.jpg|300px]]
-'''2. Scope'''
-**  This instrument is used to meaure how much light is absorbed by a liquid sample. In full working condition this instrument supports a range of visible and UV wavelength of light.
-'''3. Responsibilities'''
-**  It is the responsibility of lab users to use this equipment in the manner described in this SOP.
-**  It is the responsibility of lab users to report any incidents or technical problems with the equipment
-**  It is the responsibility of the lab management to provide training, technical support and to ensure compliance with the operating procedures.
-'''4. Materials'''
-** Quartz or plastic cuvettes with a optical path of 12mm
-** The equipment has replaceable UV and visible light bulbs
-'''5. Related documents'''
-**  https://wiki.london.hackspace.org.uk/view/Spectrophotometer
-**  Software manual http://www.vtpup.cz/common/manual/PrF_biofyz_ThermoSpectronic_UV500_manual_EN.pdf
-**  User Guide https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Mdemploi/UNI050_EN%202203_%20HELIOS%20SERIES_%20USER.pdf
-'''6. Definitions'''
-**  Cuvette: A small, transparent quartz vessel. This is what a sample is put into for testing in the UV - Visible Spectrophotometer.
-'''7. Procedures'''
-** The spectrophotometer should be started with good time before its required as the initialization procedure takes ~15 mins
-** The user should ensure that the sample cuvette is clean before it is inserted into the device
-** For OD600 selected the wavelength for 600nm.
-** run a reference sample of media only sample
-** run the sample cuvette
-** ensure the machine is switched off after use
-[[Category:Biohacking]]

Difference between revisions of "LBL04001"

Latest revision as of 20:15, 13 October 2015

Navigation menu

Search