LBL04007

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Topping up the RO water level in the Autoclave

Author: Tolland Approved by: S. Thompson SOP No. LBL04007
Signed: Signed: Effective from:
Date: Date: Last edited:

1. Purpose This describes a user maintainance procedure for the Melag EuroKlav 29V-S. 2. Scope

This should be observed every time the autoclave indicates it requires a water fill top up.

3. Responsibilities

Care should be taken to not splash water when filling.

4. Materials

The autoclave requires a supply of RO water. If the water supply level falls below a minimum level, the operator will be required to top up the system. The RO water is available from the tank under the counter. The autoclave is filled into the tank from the top of the machine.


If there are any problems, please email the [mailti:hello@biohackspace.org BSO]


5. Related documents

None


6. Definitions

none


7. Procedures

7.1 Top up water level

If the water level falls below a particular level, a message will indicate that a fill is required


7.1.1 Sterilize the load using the Universal Programme (28 psi/134 C/30 mins)


7.2 Deactivation of liquid and solid GMO waste ==

7.2.1 Transport the waste to the autoclave in a non-drip autoclavable tray 7.2.2 Ensure there are no folds or containers which can trap air 7.2.3 Sterilize the load using the Universal-Programme (2.1 bar/134 C/15 mins) 7.2.4 once the programme has run, the waste should be transferred to the chemical kill bin, for final deactivation

8. Resources

- Operating manual: http://www.eickemeyer.nl/images/products/2281/500032_Melag_Euroklav_23VS.pdf