Difference between revisions of "Project:OD600 Measurement"
m |
m |
||
| Line 23: | Line 23: | ||
use a stain that binds to a protein that is only expressed during exponential growth phase | use a stain that binds to a protein that is only expressed during exponential growth phase | ||
| + | |||
| + | [[Category:Biohacking]] | ||
Revision as of 09:58, 3 September 2014
The idea is to take a 600 nm source, (or a source including 600 nm and a BP filter) and a level detector, and calibrate it to match 0.4 at OD600 on a proper spectrophotometer..
At the moment the main issue is that the LDR (light dependent resistor) only sees about 20 ohms of difference between the initial inoculation and the mid-log phase, which shows as 0.4 OD600 on a spectrophotometer.
If you don't have access to a spectrophotometer, (or even if you do), this should be a convenient way to estimate OD600 values for your sample
A secondary project is to cast some 10mm x 10mm resin blocks to be used for calibration against a system that takes a cuvette.
Here is a document describing various methods for calibrating spectrophotometers;
http://www.perkinelmer.co.uk/CMSResources/Images/44-136839TCH_Validating_UV_Visible.pdf
alternative ideas
use a stain that binds to a protein that is only expressed during exponential growth phase