Difference between revisions of "User:Tolland"
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| − | Is a biohacker also working on | + | Is a [https://biohackspace.org biohacker] also working on microcontroller and biolab automation projects. Is interesting in learning new fabrication techniques and tools. |
contact email: tom@limepepper.co.uk | contact email: tom@limepepper.co.uk | ||
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This is a video I made of the interior of a single cell dinoflagellate using a Samsung S2 cameraphone and a OMAX microscope from the Dinos in the biolab | This is a video I made of the interior of a single cell dinoflagellate using a Samsung S2 cameraphone and a OMAX microscope from the Dinos in the biolab | ||
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| − | + | [https://www.youtube.com/watch?v=LUX4PpBc7nk Dinos in the biolab] | |
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| + | [[File:2023-09-15 20-35.png|400px]] | ||
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| + | Bacteria Dividing (12hour time lapse) | ||
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| + | [https://www.youtube.com/watch?v=k4fnn5QLRzg Bacteria dividing under microscope with focus controlled by stepper] | ||
| + | [[File:2023-09-15 20-32.png|400px]] | ||
===Welding=== | ===Welding=== | ||
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| − | + | == Biolab Equipment == | |
I made a new keypad for this PCR machine (it works honest!); | I made a new keypad for this PCR machine (it works honest!); | ||
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[[File:15178514288 b4db2555fb c.jpg|400px]] | [[File:15178514288 b4db2555fb c.jpg|400px]] | ||
| − | == [[Project:AlgaeIlluminator|Algae Illuminator Lamp]] == | + | === [[Project:AlgaeIlluminator|Algae Illuminator Lamp]] === |
Bio luminescent algeo require a "training" cycle of 12 hours of darkness, followed by 12 hours of light. They will then produce the maximum light output when agitated when in their "night time" phase. This is a device to optimally illuminate a flask of dinoflaggelates using an arduino controlled led lamp array. | Bio luminescent algeo require a "training" cycle of 12 hours of darkness, followed by 12 hours of light. They will then produce the maximum light output when agitated when in their "night time" phase. This is a device to optimally illuminate a flask of dinoflaggelates using an arduino controlled led lamp array. | ||
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</gallery> | </gallery> | ||
| − | == [[Project:Biobot|Biobot data logger and IRC bot]] == | + | === [[Project:Biobot|Biobot data logger and IRC bot]] === |
Biobot is a raspberry pi based data logger and IRC bot. | Biobot is a raspberry pi based data logger and IRC bot. | ||
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http://webchat.freenode.net/?channels=london-biohackers | http://webchat.freenode.net/?channels=london-biohackers | ||
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| − | == [[Project:Biohackers microscope|Biohacker microscope]] == | + | === [[Project:Biohackers microscope|Biohacker microscope]] === |
This is a project to create a cheap mount for mobile phone cameras, and to transmit the live image using an android app to a desktop computer; | This is a project to create a cheap mount for mobile phone cameras, and to transmit the live image using an android app to a desktop computer; | ||
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</gallery> | </gallery> | ||
| − | == Inverted microscope == | + | === Inverted microscope === |
A proof of concept of a cheap researcher microscope in an inverted configuration using a makerbeam frame system; | A proof of concept of a cheap researcher microscope in an inverted configuration using a makerbeam frame system; | ||
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</gallery> | </gallery> | ||
| − | == [[Project:Nasal flow data logger|Nasal flow data logger]] == | + | === [[Project:Nasal flow data logger|Nasal flow data logger]] === |
A simple device to measure the relative left/right nasal flow using an arduino and differential air pressure component. | A simple device to measure the relative left/right nasal flow using an arduino and differential air pressure component. | ||
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</gallery> | </gallery> | ||
| − | == [[Project:shaker platform|Shaker Platform]] == | + | === [[Project:shaker platform|Shaker Platform]] === |
many techniques for culturing microbes benefit from continuous agitation. This is a project to build a shaker platform out of readily available parts and drive the mechanism using the plate turner in a microwave over. | many techniques for culturing microbes benefit from continuous agitation. This is a project to build a shaker platform out of readily available parts and drive the mechanism using the plate turner in a microwave over. | ||
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| − | == [[Project:OD600|OD600 handheld unit]] == | + | === [[Project:OD600|OD600 handheld unit]] === |
This is a project to create a cheap hand held unit to determine the [http://bitesizebio.com/1005/what-you-need-to-know-about-od600/ OD600] level of a biological sample. Typically this is used for determining [http://en.wikipedia.org/wiki/Bacterial_growth#Phases mid-log phase] of bacterial growth in molecular biology. | This is a project to create a cheap hand held unit to determine the [http://bitesizebio.com/1005/what-you-need-to-know-about-od600/ OD600] level of a biological sample. Typically this is used for determining [http://en.wikipedia.org/wiki/Bacterial_growth#Phases mid-log phase] of bacterial growth in molecular biology. | ||
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| − | + | == Laser Cutting == | |
3d printed pi case from design on instructables | 3d printed pi case from design on instructables | ||
[[File:14655455469 0e2d12dd10 b.jpg|400px]] | [[File:14655455469 0e2d12dd10 b.jpg|400px]] | ||
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| + | ===Lab Automation=== | ||
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| + | I made a automatic pipettor with a stepper motor, the linear bearing was done on the 3-in-one lathe | ||
| + | {{#ev:youtube|pEgEQ2Acyv8}} | ||
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| + | === [[Project:Automated Pipettor|Automated Pipettor for robot arm]] === | ||
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| + | The hackspace has a staubli robot arm. This is a project to create a automated pipettor driven by a stepper motor | ||
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| + | <gallery> | ||
| + | File:Linearactuatorforpipettor.jpg | ||
| + | </gallery> | ||
| + | |||
| + | We have some components that can be used to improve this | ||
Latest revision as of 19:38, 15 September 2023
Is a biohacker also working on microcontroller and biolab automation projects. Is interesting in learning new fabrication techniques and tools.
contact email: tom@limepepper.co.uk
Stuff I made list
Video Microscopy
This is a video I made of the interior of a single cell dinoflagellate using a Samsung S2 cameraphone and a OMAX microscope from the Dinos in the biolab
Bacteria Dividing (12hour time lapse)
Bacteria dividing under microscope with focus controlled by stepper
Welding
MIG welded candlestick (done at the "One little girl and a can of gasoline" studio over in Clare street)
This trolley was originally MIG welded from steel tube for transporting a laser, but it's now donated to the space in the PCB area.
Biolab Equipment
I made a new keypad for this PCR machine (it works honest!);
For a while we had some bio-luminescent algae in the biolab, and this was their illumination box as they are photosynthetic algae
Algae Illuminator Lamp
Bio luminescent algeo require a "training" cycle of 12 hours of darkness, followed by 12 hours of light. They will then produce the maximum light output when agitated when in their "night time" phase. This is a device to optimally illuminate a flask of dinoflaggelates using an arduino controlled led lamp array.
Biobot data logger and IRC bot
Biobot is a raspberry pi based data logger and IRC bot.
you can talk to it in #london-biohackers on freenode
http://webchat.freenode.net/?channels=london-biohackers
Biohacker microscope
This is a project to create a cheap mount for mobile phone cameras, and to transmit the live image using an android app to a desktop computer;
Inverted microscope
A proof of concept of a cheap researcher microscope in an inverted configuration using a makerbeam frame system;
Nasal flow data logger
A simple device to measure the relative left/right nasal flow using an arduino and differential air pressure component.
Shaker Platform
many techniques for culturing microbes benefit from continuous agitation. This is a project to build a shaker platform out of readily available parts and drive the mechanism using the plate turner in a microwave over.
OD600 handheld unit
This is a project to create a cheap hand held unit to determine the OD600 level of a biological sample. Typically this is used for determining mid-log phase of bacterial growth in molecular biology.
I'd also like to resin cast some calibration blocks, so I can compare between equipment in a standard way;
http://www.myfluxcapacitor.com/plastic-resin-casting-guide
Laser Cutting
3d printed pi case from design on instructables
Lab Automation
I made a automatic pipettor with a stepper motor, the linear bearing was done on the 3-in-one lathe {{#ev:youtube|pEgEQ2Acyv8}}
Automated Pipettor for robot arm
The hackspace has a staubli robot arm. This is a project to create a automated pipettor driven by a stepper motor
We have some components that can be used to improve this