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* The bands are within the bounds of the ladder this time. | * The bands are within the bounds of the ladder this time. | ||
* Bands on lanes 3, 4, and 6 are more blurry than bands on lanes 2 and 7. One exciting theory is that bands on those lanes actually worked, and the blurriness is because of the 200 base-pair difference between the two amelogenin sequences; whereas the bands on lanes 2 and 7 contain un-used primer and master mix, which is a) shorter sequences and b) less well-defined (presumably because there are various different lengths). | * Bands on lanes 3, 4, and 6 are more blurry than bands on lanes 2 and 7. One exciting theory is that bands on those lanes actually worked, and the blurriness is because of the 200 base-pair difference between the two amelogenin sequences; whereas the bands on lanes 2 and 7 contain un-used primer and master mix, which is a) shorter sequences and b) less well-defined (presumably because there are various different lengths). | ||
= Future = | |||
* Try to get the agar more consistent | |||
* Electrophorese for 20 or 30 minutes, but no longer | |||