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Project:Sex typing with amelogenin/results-20120212: Difference between revisions
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Project:Sex typing with amelogenin/results-20120212 (view source)
Revision as of 23:21, 12 February 2012
, 12 February 2012Couple more discussion points and clarified lanes.
m (→Discussion) |
(Couple more discussion points and clarified lanes.) |
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=== Gel lanes (viewed with the gel oriented so the lanes are at the top) === | === Gel lanes (viewed with the gel oriented so the lanes are at the top) === | ||
# Nothing | # Nothing | ||
# | # master mix and primers, no template and 30 ul of buffer | ||
# | # master mix, primers, and template (from top of tube) | ||
# | # master mix, primers, and template (from bottom of tube) | ||
# Ladder, 6ul | # Ladder, 6ul | ||
# | # master mix (old one), primers, and template (from top of tube) | ||
# | # master mix (old one), primers, and template (from bottom of tube) | ||
# Nothing | # Nothing | ||
| Line 99: | Line 99: | ||
* Try to get the agar more consistent | * Try to get the agar more consistent | ||
* Electrophorese for 20 or 30 minutes, but no longer | * Electrophorese for 20 or 30 minutes, but no longer | ||
* Chances are we *haven't* ruined our old master mix, which is great (£) | |||
