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(Couple more discussion points and clarified lanes.) |
m (→DNA extraction) |
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| Line 33: | Line 33: | ||
* place on ice for 1 minute | * place on ice for 1 minute | ||
* centrifuge for 30 seconds | * centrifuge for 30 seconds | ||
* transfer 200ul of supernatant to fresh tube. This contains DNA. | * transfer 200ul of supernatant to fresh tube. This contains DNA. (Note I didn't do this step -- instead I took liquid from the top of the tube or the bottom of the tube, depending on the lane.) | ||
== Protocol == | == Protocol == | ||