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Project:Sex typing with amelogenin/results-20120212: Difference between revisions
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Project:Sex typing with amelogenin/results-20120212 (view source)
Revision as of 12:40, 14 February 2012
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* Chances are we *haven't* ruined our old master mix, which is great (£) | * Chances are we *haven't* ruined our old master mix, which is great (£) | ||
* We should aliquot our master mixes so we don't have to put them through any more thaw / freeze cycles. | * We should aliquot our master mixes so we don't have to put them through any more thaw / freeze cycles. | ||
* We may have too much template (surprisingly, this can be a problem) | |||
* Do we need hot start? | |||
Basically I think we are screwing up the PCR stage: using the wrong temperatures, too-short timings, or whatever. Here is a paper on sex typing with amelogenin: | Basically I think we are screwing up the PCR stage: using the wrong temperatures, too-short timings, or whatever. Here is a paper on sex typing with amelogenin: | ||
