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Project:Sex typing with amelogenin/results-20120301: Difference between revisions
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Project:Sex typing with amelogenin/results-20120301 (view source)
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More photos of these results are available here: [[http://lardcave.net/biohacking/2012-03-02/]] | More photos of these results are available here: [[http://lardcave.net/biohacking/2012-03-02/]] | ||
The descriptions below refer to gel lanes from the top downwards. Sorry, Rafael, but I didn't bother to rotate them this time :| | |||
== Experiment 1 == | == Experiment 1 == | ||
Visualised the previous night's PCR run on 1% agarose gel with 10ul/100ml ethidium bromide stock solution. | Visualised the previous night's PCR run on 1% agarose gel with 10ul/100ml ethidium bromide stock solution. | ||
[[File:2012-02-29-results-1.JPG|200px|thumb|none|Results of experiment 1 (Nicholas, Helena, Mike, Tonderai)]] | [[File:2012-02-29-results-1.JPG|200px|thumb|none|Results of experiment 1 (Empty lane, ladder, Nicholas, Helena, Mike, Tonderai)]] | ||
It is hard to see, but it almost looks like lane 4 (Tonderai? Or was it Mike?) is almost right -- there seem to be two distinct bands. | It is hard to see, but it almost looks like lane 4 (Tonderai? Or was it Mike?) is almost right -- there seem to be two distinct bands. | ||
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I used the old master mix, as we have run out of the new stuff. | I used the old master mix, as we have run out of the new stuff. | ||
[[File:2012-03-01-results-1.JPG|200px|thumb|none|Results of experiment 1 (Nicholas - meat tenderiser, Nicholas - meat tenderiser, Nicholas - heat to lyse)]] | [[File:2012-03-01-results-1.JPG|200px|thumb|none|Results of experiment 1 (Empty lane, ladder, Nicholas - meat tenderiser, Nicholas - meat tenderiser, Nicholas - heat to lyse)]] | ||
Once again, two distinct bands are visible, but they don't seem to be in the right places on the gel. | Once again, two distinct bands are visible, but they don't seem to be in the right places on the gel. | ||
