179
edits
Project:Sex typing with amelogenin/results-20120301: Difference between revisions
From London Hackspace Wiki
Project:Sex typing with amelogenin/results-20120301 (view source)
Revision as of 21:21, 2 March 2012
, 2 March 2012no edit summary
mNo edit summary |
mNo edit summary |
||
| Line 15: | Line 15: | ||
The protocol was as usual (see previous 4 or so notes), using the meat tenderiser method. He was careful to: | The protocol was as usual (see previous 4 or so notes), using the meat tenderiser method. He was careful to: | ||
* Ensure the meat tenderiser / saliva solution was well mixed, with a 1-2 minute "sitting time" for the enzyme to take effect | |||
* Ensure the alcohol was mixed with the tenderiser solution, in order to precipitate out the most DNA | |||
* Ensure that the DNA, when extracted from the alcohol / saliva / tenderiser solution (yuck), was well dried, by heating for 5 min at 55 degrees. | |||
I used the old master mix, as we have run out of the new stuff. | I used the old master mix, as we have run out of the new stuff. | ||
