179
edits
mNo edit summary |
m (→Experiment 2) |
||
| Line 20: | Line 20: | ||
I used the old master mix, as we have run out of the new stuff. | I used the old master mix, as we have run out of the new stuff. | ||
Visualised on 1% agarose gel with 6ul/100ml ethidium bromide solution. NB we have previously been using 5ul/100ml. Based on these results I am not convinced that more EtBr is at all helpful -- I noticed no difference in brightness between these two runs. | |||
[[File:2012-03-01-results-1.JPG|200px|thumb|none|Results of experiment 1 (Empty lane, ladder, Nicholas - meat tenderiser, Nicholas - meat tenderiser, Nicholas - heat to lyse)]] | [[File:2012-03-01-results-1.JPG|200px|thumb|none|Results of experiment 1 (Empty lane, ladder, Nicholas - meat tenderiser, Nicholas - meat tenderiser, Nicholas - heat to lyse)]] | ||