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Thermal Cycler: Difference between revisions
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The UI is, er, interesting. After spending some time with the manual I think I have it figured out, but it's definitely something that's going to need training and / or practice to learn to use. Over time, we should try to build up a cheat sheet on this page. | The UI is, er, interesting. After spending some time with the manual I think I have it figured out, but it's definitely something that's going to need training and / or practice to learn to use. Over time, we should try to build up a cheat sheet on this page. | ||
Upgrades: If someone can build a heated lid, it could save us a degree of faff later on. Basically, it would be a metal block heated to approx 95 degrees (+/- 5 degrees should be fine) that rests on the lids of the tubes while the machine is running. | |||
== How to use it: == | |||
Soak (keep at a constant temperature): | |||
*Press File - 1 - Enter | |||
*Enter temperature | |||
PCR: | |||
*Press File - 4 - Enter | |||
*Enter temperature for denaturation step | |||
*Enter time for denaturation step | |||
*Enter temperature for annealing step | |||
*Enter time for annealing step | |||
*Enter temperature for extension step | |||
*Enter time for extension step | |||
*For 4th step enter 0°C and 0 time to signal end of process | |||
*Enter number of cycles | |||
