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Thermal Cycler: Difference between revisions
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→How to use it:
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== How to use it: == | == How to use it: == | ||
I strongly suggest you RTFM before following these instructions. Even if they're 100% correct, it's easy to get lost in the UI. The interface is quite logical and comprehensible when you're used to it, but it's bafflingly opaque to first-time users! | |||
Soak (keep at a constant temperature): | Soak (keep at a constant temperature): | ||
Start with the UI saying "STEP to enter file 1" (or file 4, or whatever). | |||
*Press File - 1 - Enter | *Press File - 1 - Enter | ||
*Enter temperature | *Enter temperature and press Enter | ||
*Press YES to start the program. | |||
*To pause the soak, press NO. Press it again to stop the soak. | |||
PCR: | PCR: | ||
*Press File - 4 - Enter | *Press File - 4 - Enter | ||
*Enter temperature for denaturation step | *Enter temperature for denaturation step and press Enter. Press Clear if you make a mistake. | ||
*Enter time for denaturation step | *Enter time for denaturation step in minutes (enter) and seconds (enter). | ||
*Enter temperature for annealing step | *Enter temperature for annealing step | ||
*Enter time for annealing step | *Enter time for annealing step | ||
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*Enter time for extension step | *Enter time for extension step | ||
*For 4th step enter 0°C and 0 time to signal end of process | *For 4th step enter 0°C and 0 time to signal end of process | ||
*Enter number of cycles | *Select "no" for "Auto segment extension" (the cursor should be on No by default, so just press Enter). | ||
*Enter number of cycles and press Enter. | |||
*For "link to stored file", you can do one of two things. If you enter 0, the thermocycler will shut off heating and cooling to the block after PCR, which means it will gradually fall to room temperature. Alternatively, if you enter 20, the thermocycler will run a 4 degree soak file after PCR -- equivalent to putting the samples in a refrigerator. (The magic number 20 refers to a soak program we previously saved. Don't overwrite it!) | |||
