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Thermal Cycler: Difference between revisions
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== How to use it: == | == How to use it: == | ||
I strongly suggest you RTFM before following these instructions. Even if they're 100% correct, it's easy to get lost in the UI. The interface is quite logical and comprehensible when you're used to it, but it's bafflingly opaque to first-time users! | I strongly suggest you RTFM before following these instructions. Even if they're 100% correct, it's easy to get lost in the UI. The interface is quite logical and comprehensible when you're used to it, but it's bafflingly opaque to first-time users! The manual is stored in the lab - read it if you want to find out how to chain programs together to create a method. | ||
=== Setting parameters from scratch === | |||
Soak (keep at a constant temperature): | Soak (keep at a constant temperature): | ||
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*Enter number of cycles and press Enter. | *Enter number of cycles and press Enter. | ||
*For "link to stored file", you can do one of two things. If you enter 0, the thermocycler will shut off heating and cooling to the block after PCR, which means it will gradually fall to room temperature. Alternatively, if you enter 20, the thermocycler will run a 4 degree soak file after PCR -- equivalent to putting the samples in a refrigerator. (The magic number 20 refers to a soak program we previously saved. Don't overwrite it!) | *For "link to stored file", you can do one of two things. If you enter 0, the thermocycler will shut off heating and cooling to the block after PCR, which means it will gradually fall to room temperature. Alternatively, if you enter 20, the thermocycler will run a 4 degree soak file after PCR -- equivalent to putting the samples in a refrigerator. (The magic number 20 refers to a soak program we previously saved. Don't overwrite it!) | ||
=== Running stored files === | |||
* To run a stored file, press File -> [file number] -> Enter -> Start | |||
* If you want to run a method, just run the first file and it will run the whole method for you | |||
=== Stored files === | |||
==== Program for partially successful PCR run on the [[Public_Biobrick#14-15.2F9:_Hackspace 14th sept]] ==== | |||
