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Project:Plant species testing: Difference between revisions
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→Repeat without PCR (30/7/12):
Mycoplasma (talk | contribs) |
Mycoplasma (talk | contribs) |
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* Add 70% isopropanol, store at -20° overnight. | * Add 70% isopropanol, store at -20° overnight. | ||
* Centrifuge at 5000rpm for 40 mins. Isopropanol removed, pellet air dried for 20 mins, then resuspended in autoclaved H2O. | * Centrifuge at 5000rpm for 40 mins. Isopropanol removed, pellet air dried for 20 mins, then resuspended in autoclaved H2O. | ||
* 23ul of sample + 2ul of loading dye/glycerol mix electrophoresed at 80V. No staining observed. | * 23ul of sample + 2ul of loading dye/glycerol mix electrophoresed at 80V for 30 mins. No staining observed. | ||
Conclusion: extraction inadequate again. We also ran liquified pea alone with no other reagents other than autoclaved water - observed a smear. | Conclusion: extraction inadequate again. We also ran liquified pea alone with no other reagents other than autoclaved water - observed a smear. | ||
Email and photos here. | Email and photos here. | ||
