Project:Plant species testing: Difference between revisions

From London Hackspace Wiki

Line 64: Line 64:
* 23ul of sample + 2ul of loading dye/glycerol mix electrophoresed at 80V for 30 mins. No staining observed.
* 23ul of sample + 2ul of loading dye/glycerol mix electrophoresed at 80V for 30 mins. No staining observed.


Conclusion: extraction inadequate again. We also ran liquified pea alone with no other reagents other than autoclaved water - observed a smear.
Conclusion: Extraction inadequate again. (Probable cause was that sample contained plant matter, hence pellet was this not DNA. We also ran liquified pea alone with no other reagents other than autoclaved water - observed a smear.


Email and photos here.
Email and photos here.
927

edits