Anonymous

Electrophoresis Stains: Difference between revisions

From London Hackspace Wiki
Added SYBR Safe
(Copy of lhs-biohacking Message-Id: <175792F6-B834-4DC5-884F-6FFF17910441@lardcave.net>)
 
(Added SYBR Safe)
Line 47: Line 47:
* Cost, total: 5p per gel, or 0.5p per gel.
* Cost, total: 5p per gel, or 0.5p per gel.
* Other considerations: About 100 times less sensitive than EtBr (100ng DNA at most sensitive, some reports put it at no less than 500ng => won't be able to see the ladder). Post-staining is annoying.
* Other considerations: About 100 times less sensitive than EtBr (100ng DNA at most sensitive, some reports put it at no less than 500ng => won't be able to see the ladder). Post-staining is annoying.
== Option 8: SYBR Safe ==
* Info: https://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/DNA-RNA-Purification-Analysis/Nucleic-Acid-Gel-Electrophoresis/DNA-Stains/SYBR-Safe.html
* Cost of product: £44.50 for 400ul, 5ul per gel, so £0.55 per gel
* Total cost: 55p per gel.


== Discussion ==
== Discussion ==