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Electrophoresis Stains: Difference between revisions
From London Hackspace Wiki
Fixed SafeWhite error (it's per reaction, not per gel)
(Added SYBR Safe) |
(Fixed SafeWhite error (it's per reaction, not per gel)) |
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== Option 1: SafeWhite == | == Option 1: SafeWhite == | ||
* Info: http://nbsbio.co.uk/product.asp?pID=6241&cID=71 | * Info: http://nbsbio.co.uk/product.asp?pID=6241&cID=71 | ||
* Cost of product: £40 for 1ml. 2ul per | * Cost of product: £40 for 1ml. 2ul per reaction, therefore 40 / (1000 / 2) = £0.08 a reaction | ||
* Cost, total: 8p per gel | * Cost, total: 8p per reaction, assuming 4 reactions per gel = 32p per gel. | ||
* Other considerations: must be refrigerated (4C). | * Other considerations: must be refrigerated (4C). | ||
== Option 2: SafeWhite in bulk == | == Option 2: SafeWhite in bulk == | ||
* Info: http://nbsbio.co.uk/product.asp?pID=12207&cID=71 | * Info: http://nbsbio.co.uk/product.asp?pID=12207&cID=71 | ||
* Cost of product: £175 for 5ml. 2ul per | * Cost of product: £175 for 5ml. 2ul per reaction, therefore 175 / (5000 / 2) = £0.07 a sample | ||
* Cost, total: 7p per gel | * Cost, total: 7p per reaction, assuming 4 reactions per gel = 28p per gel. | ||
* Other considerations: As above. | * Other considerations: As above. | ||
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## We can't use SYBR Green + Taq to visualise ladders directly | ## We can't use SYBR Green + Taq to visualise ladders directly | ||
## Because we won't be running a ladder mixed with the same dye (at best, we'd mix the ladder with SYBR green after PCR), we won't know if we screwed up the visualisation or if the reaction didn't work (this problem is what led us to ethidium bromide in the first place). | ## Because we won't be running a ladder mixed with the same dye (at best, we'd mix the ladder with SYBR green after PCR), we won't know if we screwed up the visualisation or if the reaction didn't work (this problem is what led us to ethidium bromide in the first place). | ||
# Even with disposal costs, ethidium bromide isn't that bad. It's | # Even with disposal costs, ethidium bromide isn't that bad. It's less expensive than SafeWhite (for 2 or more reactions per gel), but, to be fair, that's still not a significant difference (10p or so). If we handle EtBr properly and dispose of it correctly, we should be fine. | ||
# For £40, we may as well check out SafeWhite. | # For £40, we may as well check out SafeWhite. It's not particularly cheap, and we will still have to retain LabWaste for waste disposal, but we won't need them to dispose of gels, so we won't need them to come as frequently. | ||
