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Project:Plant species testing: Difference between revisions
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→Chelex extraction method - 17 and 18/7/12
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* 100ml of agarose solution: 1 gram of agarose powder + 10ml of 10x TBE + 90ml purified water. | * 100ml of agarose solution: 1 gram of agarose powder + 10ml of 10x TBE + 90ml purified water. | ||
* Microwaved the agarose, cooled to 50 degrees, added 5ul of ethidium bromide stock solution | * Microwaved the agarose, cooled to 50 degrees, added 5ul of ethidium bromide stock solution. (Should be 2ul EtBr to 40ml gel) | ||
* Poured agarose into mould and waited to cool, then covered with 1% TBE. | * Poured agarose into mould and waited to cool, then covered with 1% TBE. | ||
* Put ladder into far well, template into near well | * Put ladder into far well, template into near well | ||
