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Project:Plant species testing: Difference between revisions

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Project:Plant species testing (view source)

Revision as of 18:34, 22 September 2012

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→‎Chelex extraction method - 17 and 18/7/12
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Electrophoresis:
Electrophoresis:


* 100ml of agarose solution: 1 gram of agarose powder + 10ml of 10x TBE + 90ml purified water.
* Electrophoresed for 1 hr on an 100ml 1% agarose gel - started at 80V, settled at around 130V
* Microwaved the agarose, cooled to 50 degrees, added 5ul of ethidium bromide stock solution. (Should be 2ul EtBr to 40ml gel)
* Poured agarose into mould and waited to cool, then covered with 1% TBE.
* Put ladder into far well, template into near well
* Electrophoresed for 1 hr - started at 80V, settled at around 130V
* Visualised under UV light through orange filter - did not see any bands from the template, but ladder was visible, indicating PCR failed.
* Visualised under UV light through orange filter - did not see any bands from the template, but ladder was visible, indicating PCR failed.


Mycoplasma
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