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Project:Plant species testing: Difference between revisions
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Project:Plant species testing (view source)
Revision as of 20:01, 22 September 2012
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We aim to extract DNA from plants, then PCR out a sequence and run it on a gel to test and refine our DNA extraction / PCR technique. First project is to test for peas. Inspiration and primers are in this [http://www.epochlifescience.com/Product/SpinColumn/EconoSpin%20Plant%20Seed%20gDNA.pdf paper]. We are using two methods of extraction. 1st attempt with a chelex protocol, 2nd with alcohol precipitation. | We aim to extract DNA from plants, then PCR out a sequence and run it on a gel to test and refine our DNA extraction / PCR technique. First project is to test for peas. Inspiration and primers are in this [http://www.epochlifescience.com/Product/SpinColumn/EconoSpin%20Plant%20Seed%20gDNA.pdf paper]. We are using two methods of extraction. 1st attempt with a chelex protocol, 2nd with alcohol precipitation. If you're trying to repeat any of these and not sure about the procedure, see the links on the front page. | ||
== Chelex extraction method - 17 and 18/7/12 == | == Chelex extraction method - 17 and 18/7/12 == | ||
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* Interface removed and as much liquid as possible removed from this | * Interface removed and as much liquid as possible removed from this | ||
* Pellet dried at 60°C in thermocycler. | * Pellet dried at 60°C in thermocycler. | ||
* EDIT - what happened then...? No evidence on list. | |||
