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Project:Plant species testing: Difference between revisions
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→Repeat without PCR (30/7/12):
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* Centrifuge at 5000rpm for 40 mins. Isopropanol removed, pellet air dried for 20 mins, then resuspended in autoclaved H2O. | * Centrifuge at 5000rpm for 40 mins. Isopropanol removed, pellet air dried for 20 mins, then resuspended in autoclaved H2O. | ||
* 23ul of sample + 2ul of loading dye/glycerol mix electrophoresed at 80V for 30 mins. No staining observed. | * 23ul of sample + 2ul of loading dye/glycerol mix electrophoresed at 80V for 30 mins. No staining observed. | ||
[[File:Gel_run_31_7.jpg|left|400px|thumb|none|Gel run 10th sept]] | |||
'''From left:''' | |||
*1: 250-10000bp ladder (from hackspace). Ladder [http://www.taq-dna.com/rich_files/attachments/DNA_Ladders_DNA_Weight_markers_DNA_leiter/1000_bp_1kb_DNA_Ladder.pdf key] | |||
*2: Positive control PCR product. | |||
*3: Mike PCR product | |||
*4: Simon PCR product | |||
*5: Will PCR product | |||
*6: MF1 | |||
*7: N.H / N.1+ | |||
Conclusion: Extraction inadequate again. (Probable cause was that sample contained plant matter, hence pellet was this not DNA. We also ran liquified pea alone with no other reagents other than autoclaved water - observed a smear. | Conclusion: Extraction inadequate again. (Probable cause was that sample contained plant matter, hence pellet was this not DNA. We also ran liquified pea alone with no other reagents other than autoclaved water - observed a smear. | ||
