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Project:Plant species testing: Difference between revisions
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* Shake of meat tenderiser added to break down proteins | * Shake of meat tenderiser added to break down proteins | ||
* Centrifuged and supernatant removed - solids discarded. | * Centrifuged and supernatant removed - solids discarded. | ||
* 5ml of 70% added to 10ml of template for precipitation | * 5ml of 70% isopropanol added to 10ml of template for precipitation | ||
* Interface removed and as much liquid as possible removed from this | * Interface removed and as much liquid as possible removed from this | ||
* Pellet dried at 60°C in thermocycler. | * Pellet dried at 60°C in thermocycler. | ||
* EDIT - what happened then...? No evidence on list. | * EDIT - what happened then...? No evidence on list. | ||
===DNA extraction (16/10/12): === | |||
*12g peas crushed with pestle in 50ml dH20 + 0.5g salt | |||
*20ml triton mix added and left for 10 mins | |||
*1 volume of 70% isopropanol added. No precipitate formed so discarded. | |||
Conclusion - either peas not crushed properly, or triton mix ineffective. | |||
*small quantity of bacon crushed with pestle in 30ml dH20 + 0.5g salt | |||
*Resulting mix split into two, 10ml triton mix added to one and 10ml ordinary detergent added to the other. Left for 10 mins | |||
*1 volume of 70% isopropanol added to each. Precipitate observed in both. Precipitate removed with pipette and frozen. | |||
Will try running on gel to test extraction. | |||
