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Project:Plant species testing: Difference between revisions
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Saw a faint, blurry band, running ahead of the ladder. Not sure what this is, as genomic DNA should be behind the ladder if not still in the well. | Saw a faint, blurry band, running ahead of the ladder. Not sure what this is, as genomic DNA should be behind the ladder if not still in the well. | ||
====Extraction and PCR 27 Jul 12 ==== | |||
Failed. Description on [https://groups.google.com/forum/?fromgroups=#!searchin/london-biohackers/not-so-/london-biohackers/P8A_Mu7Y8os/_a4orNt5oosJ list] | |||
==== Repeat without PCR: 30/7/12 ==== | ==== Repeat without PCR: 30/7/12 ==== | ||
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Conclusion: none of the runs prove we have DNA in the gel, indicating our extractions failed. | Conclusion: none of the runs prove we have DNA in the gel, indicating our extractions failed. | ||
====Extraction and PCR 27 Jul 12 ==== | |||
Failed. Description on [https://groups.google.com/forum/?fromgroups=#!searchin/london-biohackers/not-so-/london-biohackers/P8A_Mu7Y8os/_a4orNt5oosJ list] | |||
===Repeat without PCR (30/7/12): === | ===Repeat without PCR (30/7/12): === | ||
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*5: Sample of liquified pea with no other reagents added apart from autoclaved water. | *5: Sample of liquified pea with no other reagents added apart from autoclaved water. | ||
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Conclusion: Isopropanol extractions failed because pellet contained too much plant matter - did not filter properly. | Conclusion: Isopropanol extractions failed because pellet contained too much plant matter - did not filter properly. | ||
