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Project:Plant species testing: Difference between revisions
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→Repeat without PCR (30/7/12):
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* Add proteinease K (25 ul of 1mg / ml solution to 80 ul of pea template) - incubate at 50°C for 10 mins (action of proteinase k) then 94 C for 10 mins (inactivation of proteinase K) | * Add proteinease K (25 ul of 1mg / ml solution to 80 ul of pea template) - incubate at 50°C for 10 mins (action of proteinase k) then 94 C for 10 mins (inactivation of proteinase K) | ||
* Add 70% isopropanol, store at -20° overnight. | * Add 70% isopropanol, store at -20° overnight. | ||
* Centrifuge at | * Centrifuge at 1400rpm for 40 mins. Isopropanol removed, pellet air dried for 20 mins, then resuspended in autoclaved H2O. | ||
* 23ul of sample + 2ul of loading dye/glycerol mix electrophoresed at 80V for 30 mins. No staining observed. | * 23ul of sample + 2ul of loading dye/glycerol mix electrophoresed at 80V for 30 mins. No staining observed. | ||
