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Project:Plant species testing: Difference between revisions
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Project:Plant species testing (view source)
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We aim to extract DNA from plants, then PCR out a sequence and run it on a gel to test and refine our DNA extraction / PCR technique. First project is to test for peas. Inspiration and primers are in this [http://www.epochlifescience.com/Product/SpinColumn/EconoSpin%20Plant%20Seed%20gDNA.pdf paper]. We are using two methods of extraction. 1st attempt with a chelex protocol, 2nd with alcohol precipitation. If you're trying to repeat any of these and not sure about the procedure, see the links on the front page. | We aim to extract DNA from plants, then PCR out a sequence and run it on a gel to test and refine our DNA extraction / PCR technique. First project is to test for peas. Expected product is 280bp. Inspiration and primers are in this [http://www.epochlifescience.com/Product/SpinColumn/EconoSpin%20Plant%20Seed%20gDNA.pdf paper]. We are using two methods of extraction. 1st attempt with a chelex protocol, 2nd with alcohol precipitation. If you're trying to repeat any of these and not sure about the procedure, see the links on the front page. | ||
So far (Oct 2012) this hasn't worked. We think this is because of the extraction process. Cuuld try Irene's protocol. (ask Will) | So far (Oct 2012) this hasn't worked. We think this is because of the extraction process. Cuuld try Irene's protocol. (ask Will) | ||
