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Project:Sex typing with amelogenin: Difference between revisions
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Project:Sex typing with amelogenin (view source)
Revision as of 23:14, 24 April 2014
, 24 April 2014→29th Feb 2012
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== 29th Feb 2012 == | == 29th Feb 2012 == | ||
This week we ran a gel for the results of last week's work. Buffer was ~20g of 10xTBE diluted with ~200g of battery top-up water. Gel was made with ~1.0g of agarose in ~100g of buffer, and 5u of EthBr added. We judged how much to pour into the tray by eye, but when the wells came up very shallow, we thought maybe use the scales in future. | |||
For whatever reason, the experiment was not a success. We couldn't visualise anything. The ladder was a barely-discernible smear, and none of the other lanes was visible at all. | |||
We decided to do another run: Nicholas, Helena, Mike, and Tonderai contributed DNA for overnight PCR. | |||
== 1st March 2012 == | == 1st March 2012 == | ||
