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Project:Sex typing with amelogenin: Difference between revisions
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Project:Sex typing with amelogenin (view source)
Revision as of 23:35, 24 April 2014
, 24 April 2014→1st March 2012
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== 1st March 2012 == | == 1st March 2012 == | ||
[[/results-20120301]] | |||
Started using orange filter for gel visualizations. | |||
'''Visualised the previous night's PCR run:''' | |||
[[File:2012-02-29-results-1.JPG|200px|thumb|none|Results of experiment 1. From top: Empty lane, ladder, Nicholas, Helena, Mike, Tonderai]] | |||
It is hard to see, but it almost looks like lane 4 (Tonderai? Or was it Mike?) is almost right -- there seem to be two distinct bands. | |||
We did not cover the master mix / primers / template solution with oil. The PCR reaction vessels had condensed liquid all over the vessel, including up near the lid. I (Nicholas) theorise that the mixture evaporated, inhibiting PCR, in some cases severely. | |||
'''Attempt to remedy evaporation during PCR:''' | |||
I tried two different oils: | |||
* "Laser Lube" (Thanks to Hipster for this description) -- machine lubricant, from a squeeze bottle near a drill press | |||
* A mysterious bottle of oil I found in the biohacking box, which could be mineral oil (though it's not the same bottle that we were using before). | |||
[[File:2012-03-01-results-1.JPG|200px|thumb|none|Results of experiment 1 From top: Empty lane, ladder, Nicholas - meat tenderiser and laser lube, Nicholas - meat tenderiser and mystery oil, Nicholas - heat to lyse and mystery oil]] | |||
Once again, two distinct bands are visible, but they don't seem to be in the right places on the gel. | |||
I conclude that we can use either mystery oil or laser lube. | |||
We continued to run the gels with visualisation once every ~12 mins | |||
[[File:Gel_IMG_20120301_194156_edit.jpg|200px|thumb|none| At about 19:42, we have all six lines of the ladder and something that looks like a line in the third lane somewhere about the 3rd or 4th rung on the ladder (which is what we are hoping for)]] | |||
More details: [[/results-20120301]] | |||
== 7th March 2012 == | == 7th March 2012 == | ||
